Lines referred to as cytolines are genotypes in which the organelle (mitochondria and plastids) and nuclear genomes originate from two different natural accessions. They can be used to evaluate the impact of cytoplasmic variation and nuclear-cytoplasmic interactions on plant phenotypes. The genetic factors involved in these interactions can then be identified using F2 populations resulting from crosses between the same accessions, which are available here. 56 cytolines were generated from reciprocal crosses between the eight accessions of the core collection followed by recurrent backcrossing with the nuclear genome donor. Three successive paternal backcrosses were performed, and plants homozygous for the recurrent parent genotype were selected by genotyping with markers distributed along the genome (Roux et al. 2016 pdf+SI). The list of markers used for each cytoline is available here.